|
KMID : 1138520190220010041
|
|
Journal of Pharmacopuncture
2019 Volume.22 No. 1 p.41 ~ p.48
|
|
|
Propugnating Effect of Bark of Rhizophora mucronata Against Different Toxicants Viz Carbon Tetrachloride, Ethanol and Paracetamol on HepG 2 Cell Lines
|
|
Chitra Jairaman
Mohamed Yacoob Syed Ali
Anuradha Venkatraman
Yogananth Nagarajan
Gnanadesigan Murugesan
|
|
|
Abstract
|
|
|
sns-share facebook go Twitter go Google+ go
Original article
Propugnating Effect of Bark of Rhizophora mucronata Against Different Toxicants Viz Carbon Tetrachloride, Ethanol and Paracetamol on HepG 2 Cell Lines
Jairaman Chitra 1, Yacoob Syed Ali Mohamed 1 *, Venkatraman Anuradha 2, Nagarajan Yogananth 1, Murugesan Gnanadesigan 3
1 PG and Research Department of Biotechnology, Mohammed Sathak College of Arts & Science, Shollinganallur, Chennai-119, Tamil nadu, India
2 PG and Research Department of Biochemistry, Mohammed Sathak College of Arts & Science, Shollinganallur, Chennai-119, Tamil nadu, India
3 Department of Microbial Biotechnology, Bharathair University, Coimbatore-641046, Tamil nadu, India
* Syed Ali Mohamed Yacoob. PG and Research Department of Biotechnology, Mohamed Sathak College of Arts and Science, Sholinganallur, Chennai, India. Tel: +91-984-205-2929, +91-741-840-6385 E-mail: syedmicro555@gmail.com
[received date: 2018-09-21 / accepted date: 2019-02-11]
Abstract
Objective: The aim of the study was to evaluate the hepatoprotective activity of the bark extract (Ethanol: Water) in the ratio of (3:1) of Rhizophora mucronata (BERM) by intoxicating the HepG 2 cell lines with different toxicants viz, CCL 4 , Ethanol and Paracetamol with different concentrations of the extract were used. The HepG 2 cell lines were subjected to MTT Assay for studying the cytotoxicity.
Methods: HepG 2 cells were plated using 96 well plate in 10% bovine serum, exposed to different toxicants viz, 2% CCl 4 , 60% Ethanol and 14 mM Paracetamol respectively. The various test concentrations (18.85, 37.5, 75, 150 and 300 μg/ml) of bark extract of Rhizophora mucronata was added and incubated for 24 hours. Medium was removed after incubation period and 0.5 mg/ml MTT(3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) was added and again incubated for 4 hours at 37oC. Then MTT was removed the crystals was dissolved in DMSO and absorbance was measured at 570 nm.
Results: The result showed that dose dependent increase in percentage of viability at the doses of 18.85, 37.5, 75, 150, 300 μg/ml. The results for the CCl4 intoxicated, at 300 μg/ml of the concentration of the extract, the % of viable cells was found out to be 99.6%, for Ethanol intoxicated, 97.67%, and Paracetamol induced, 75.37%, IC50 was 21.53 μg/ml, 12.61 μg/ml and 21.42 μg/ml respectively.
Conclusion: Thus, we conclude that, the extract possesses defensive effect against different toxicants and can be used as an alternate drug for hepatotoxicity.
|
|
|
KEYWORD
|
|
|
cytotoxicity, ethanol: water (3:1) extract, toxicants, viable cells
|
|
|
FullTexts / Linksout information
|
|
|
|
|
Listed journal information
|
|
|
|